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rosettesep immunodensity-based cell separation  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc rosettesep immunodensity-based cell separation
    SF3B1 mutation effect is independent of the biological background, but its manifestation depends on the transcriptomic profile. ( A ) The number of alternative splicing events (ASEs) identified with Iso-Seq <t>separated</t> by splicing event type in all groups investigated, differentiated by the novelty class. ( B ) Overlap between significantly altered ASEs in samples with the SF3B1 mutation identified in the three data sets used (cell lines, CLL patients, or MDS patients). ( C ) Correlation of isoform usage measured by the difference in PSI from all events listed in B ; namely, events called significant in at least one of the three data sets are shown. The colors of the dots correspond to significance reached only in one set: blue indicates x -axis only; red, y -axis only; light blue, both; and gray, none (i.e., called significant in a data set absent from the graph). Pearson correlation coefficient ( R ) and associated P -value ( P ) are given. ( D ) Violin plots with boxplots show the distribution of expression values of the genes with data set–specific ASEs from C . Significant differences are marked with as follows: (***) paired, two-tailed Student's t -test P -value < 0.001, (**) P -value < 0.01, (*) P -value < 0.05, (N.S.) not significant with P -value ≥ 0.05.
    Rosettesep Immunodensity Based Cell Separation, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rosettesep+immunodensity+cell+separation/rosettesep+immunodensity+cell+separation/pmc11610591-235-12-14
    Average 90 stars, based on 1 article reviews
    rosettesep immunodensity-based cell separation - by Bioz Stars, 2026-10
    90/100 stars

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    1) Product Images from "Long-read transcriptome sequencing of CLL and MDS patients uncovers molecular effects of SF3B1 mutations"

    Article Title: Long-read transcriptome sequencing of CLL and MDS patients uncovers molecular effects of SF3B1 mutations

    Journal: Genome Research

    doi: 10.1101/gr.279327.124

    SF3B1 mutation effect is independent of the biological background, but its manifestation depends on the transcriptomic profile. ( A ) The number of alternative splicing events (ASEs) identified with Iso-Seq separated by splicing event type in all groups investigated, differentiated by the novelty class. ( B ) Overlap between significantly altered ASEs in samples with the SF3B1 mutation identified in the three data sets used (cell lines, CLL patients, or MDS patients). ( C ) Correlation of isoform usage measured by the difference in PSI from all events listed in B ; namely, events called significant in at least one of the three data sets are shown. The colors of the dots correspond to significance reached only in one set: blue indicates x -axis only; red, y -axis only; light blue, both; and gray, none (i.e., called significant in a data set absent from the graph). Pearson correlation coefficient ( R ) and associated P -value ( P ) are given. ( D ) Violin plots with boxplots show the distribution of expression values of the genes with data set–specific ASEs from C . Significant differences are marked with as follows: (***) paired, two-tailed Student's t -test P -value < 0.001, (**) P -value < 0.01, (*) P -value < 0.05, (N.S.) not significant with P -value ≥ 0.05.
    Figure Legend Snippet: SF3B1 mutation effect is independent of the biological background, but its manifestation depends on the transcriptomic profile. ( A ) The number of alternative splicing events (ASEs) identified with Iso-Seq separated by splicing event type in all groups investigated, differentiated by the novelty class. ( B ) Overlap between significantly altered ASEs in samples with the SF3B1 mutation identified in the three data sets used (cell lines, CLL patients, or MDS patients). ( C ) Correlation of isoform usage measured by the difference in PSI from all events listed in B ; namely, events called significant in at least one of the three data sets are shown. The colors of the dots correspond to significance reached only in one set: blue indicates x -axis only; red, y -axis only; light blue, both; and gray, none (i.e., called significant in a data set absent from the graph). Pearson correlation coefficient ( R ) and associated P -value ( P ) are given. ( D ) Violin plots with boxplots show the distribution of expression values of the genes with data set–specific ASEs from C . Significant differences are marked with as follows: (***) paired, two-tailed Student's t -test P -value < 0.001, (**) P -value < 0.01, (*) P -value < 0.05, (N.S.) not significant with P -value ≥ 0.05.

    Techniques Used: Mutagenesis, Alternative Splicing, Expressing, Two Tailed Test

    Related Articles

    Isolation:

    Article Title: Bystander monocytic cells drive infection-independent NLRP3 inflammasome response to SARS-CoV-2
    Article Snippet: .. Monocytes were isolated using the RosetteSep Immunodensity Cell Separation (Stem Cell Technologies cat# 15028) for human monocytes and primary monocytes were immediately used after isolation. .. For caspase-1 cleavage, a fluorescent caspase-1 inhibitor probe 660-YVAD-FMK, FLICA660, (ImmunoChemistry Technologies, cat# 9122) was added to supernatant at 24 h post-infection and incubated for 1 h at 37°C.

    Article Title: Memory-like NK cells armed with a neoepitope-specific CAR exhibit potent activity against NPM1 mutated acute myeloid leukemia
    Article Snippet: .. Healthy donor PBMC were isolated by Ficoll centrifugation and NK cells were subsequently purified using the NK Cell Isolation Kit (Miltenyi Biotec), or directly using RosetteSep immunodensity cell separation (Stem Cell Technologies). .. After purity check by flow cytometry (>95% CD56+ CD3-), the isolated human primary NK cells were incubated overnight (12-16 hours) at 37°C in RPMI10 medium supplemented with rhIL15 (1ng/mL, StemCell Technologies) to generate conventional NK (cNK) cells.

    Article Title: Bystander monocytic cells drive infection-independent NLRP3 inflammasome response to SARS-CoV-2.
    Article Snippet: .. 17 9. isolated using the RosetteSep Immunodensity Cell Separation (Stem Cell Technologies cat# 15028) for human monocytes and primary monocytes were immediately used after isolation. .. Flow cytometry assessment of caspase-1 cleavage and ASC speck formation For caspase-1 cleavage, a fluorescent caspase-1 inhibitor probe 660-YVAD-FMK, FLICA660, (ImmunoChemistry Technologies, cat# 9122) was added to supernatant at 24 h post-infection and incubated for 1 h at 37°C.

    Article Title: Venom of the Red-Bellied Black Snake Pseudechis porphyriacus Shows Immunosuppressive Potential.
    Article Snippet: .. Untouched T cells were isolated from PBMCs by RosetteSep immunodensity cell separation (STEMCELL Technologies) and coculture with allogeneic moDCs at a ratio of 10:1. .. The mixed cultures were treated with glycerol or 1 μg/mL snake venom for a further 5 days and pulsed with [3H]thymidine (1μCi/well, specific activity 5 mCi/mmoL; Amersham Life Sciences) for the final 18 h. The mean values ± SEM were plotted using GraphPad Prism version 8.0.

    Article Title: Effects of Akt Activator SC79 on Human M0 Macrophage Phagocytosis and Cytokine Production
    Article Snippet: .. Monocytes were isolated via RosetteSep Immunodensity cell separation (StemCell Technologies, Vancouver, BC, Canada). ..

    Centrifugation:

    Article Title: Memory-like NK cells armed with a neoepitope-specific CAR exhibit potent activity against NPM1 mutated acute myeloid leukemia
    Article Snippet: .. Healthy donor PBMC were isolated by Ficoll centrifugation and NK cells were subsequently purified using the NK Cell Isolation Kit (Miltenyi Biotec), or directly using RosetteSep immunodensity cell separation (Stem Cell Technologies). .. After purity check by flow cytometry (>95% CD56+ CD3-), the isolated human primary NK cells were incubated overnight (12-16 hours) at 37°C in RPMI10 medium supplemented with rhIL15 (1ng/mL, StemCell Technologies) to generate conventional NK (cNK) cells.

    Purification:

    Article Title: Memory-like NK cells armed with a neoepitope-specific CAR exhibit potent activity against NPM1 mutated acute myeloid leukemia
    Article Snippet: .. Healthy donor PBMC were isolated by Ficoll centrifugation and NK cells were subsequently purified using the NK Cell Isolation Kit (Miltenyi Biotec), or directly using RosetteSep immunodensity cell separation (Stem Cell Technologies). .. After purity check by flow cytometry (>95% CD56+ CD3-), the isolated human primary NK cells were incubated overnight (12-16 hours) at 37°C in RPMI10 medium supplemented with rhIL15 (1ng/mL, StemCell Technologies) to generate conventional NK (cNK) cells.

    Cell Isolation:

    Article Title: Memory-like NK cells armed with a neoepitope-specific CAR exhibit potent activity against NPM1 mutated acute myeloid leukemia
    Article Snippet: .. Healthy donor PBMC were isolated by Ficoll centrifugation and NK cells were subsequently purified using the NK Cell Isolation Kit (Miltenyi Biotec), or directly using RosetteSep immunodensity cell separation (Stem Cell Technologies). .. After purity check by flow cytometry (>95% CD56+ CD3-), the isolated human primary NK cells were incubated overnight (12-16 hours) at 37°C in RPMI10 medium supplemented with rhIL15 (1ng/mL, StemCell Technologies) to generate conventional NK (cNK) cells.



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    SF3B1 mutation effect is independent of the biological background, but its manifestation depends on the transcriptomic profile. ( A ) The number of alternative splicing events (ASEs) identified with Iso-Seq <t>separated</t> by splicing event type in all groups investigated, differentiated by the novelty class. ( B ) Overlap between significantly altered ASEs in samples with the SF3B1 mutation identified in the three data sets used (cell lines, CLL patients, or MDS patients). ( C ) Correlation of isoform usage measured by the difference in PSI from all events listed in B ; namely, events called significant in at least one of the three data sets are shown. The colors of the dots correspond to significance reached only in one set: blue indicates x -axis only; red, y -axis only; light blue, both; and gray, none (i.e., called significant in a data set absent from the graph). Pearson correlation coefficient ( R ) and associated P -value ( P ) are given. ( D ) Violin plots with boxplots show the distribution of expression values of the genes with data set–specific ASEs from C . Significant differences are marked with as follows: (***) paired, two-tailed Student's t -test P -value < 0.001, (**) P -value < 0.01, (*) P -value < 0.05, (N.S.) not significant with P -value ≥ 0.05.
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    Image Search Results


    SF3B1 mutation effect is independent of the biological background, but its manifestation depends on the transcriptomic profile. ( A ) The number of alternative splicing events (ASEs) identified with Iso-Seq separated by splicing event type in all groups investigated, differentiated by the novelty class. ( B ) Overlap between significantly altered ASEs in samples with the SF3B1 mutation identified in the three data sets used (cell lines, CLL patients, or MDS patients). ( C ) Correlation of isoform usage measured by the difference in PSI from all events listed in B ; namely, events called significant in at least one of the three data sets are shown. The colors of the dots correspond to significance reached only in one set: blue indicates x -axis only; red, y -axis only; light blue, both; and gray, none (i.e., called significant in a data set absent from the graph). Pearson correlation coefficient ( R ) and associated P -value ( P ) are given. ( D ) Violin plots with boxplots show the distribution of expression values of the genes with data set–specific ASEs from C . Significant differences are marked with as follows: (***) paired, two-tailed Student's t -test P -value < 0.001, (**) P -value < 0.01, (*) P -value < 0.05, (N.S.) not significant with P -value ≥ 0.05.

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    Figure Lengend Snippet: SF3B1 mutation effect is independent of the biological background, but its manifestation depends on the transcriptomic profile. ( A ) The number of alternative splicing events (ASEs) identified with Iso-Seq separated by splicing event type in all groups investigated, differentiated by the novelty class. ( B ) Overlap between significantly altered ASEs in samples with the SF3B1 mutation identified in the three data sets used (cell lines, CLL patients, or MDS patients). ( C ) Correlation of isoform usage measured by the difference in PSI from all events listed in B ; namely, events called significant in at least one of the three data sets are shown. The colors of the dots correspond to significance reached only in one set: blue indicates x -axis only; red, y -axis only; light blue, both; and gray, none (i.e., called significant in a data set absent from the graph). Pearson correlation coefficient ( R ) and associated P -value ( P ) are given. ( D ) Violin plots with boxplots show the distribution of expression values of the genes with data set–specific ASEs from C . Significant differences are marked with as follows: (***) paired, two-tailed Student's t -test P -value < 0.001, (**) P -value < 0.01, (*) P -value < 0.05, (N.S.) not significant with P -value ≥ 0.05.

    Article Snippet: Peripheral blood B cells were isolated via negative selection using RosetteSep immunodensity-based cell separation (Stemcell Technologies).

    Techniques: Mutagenesis, Alternative Splicing, Expressing, Two Tailed Test